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Gene expression analysis reveals important pathways for drought response in leaves and roots of a wheat cultivar adapted to rainfed cropping in the Cerrado biome Genet. Mol. Biol.
Poersch-Bortolon,Liane Balvedi; Pereira,Jorge Fernando; Nhani Junior,Antonio; Gonzáles,Hebert Hernán Soto; Torres,Gisele Abigail Montan; Consoli,Luciano; Arenhart,Rafael Augusto; Bodanese-Zanettini,Maria Helena; Margis-Pinheiro,Márcia.
Abstract Drought limits wheat production in the Brazilian Cerrado biome. In order to search for candidate genes associated to the response to water deficit, we analyzed the gene expression profiles, under severe drought stress, in roots and leaves of the cultivar MGS1 Aliança, a well-adapted cultivar to the Cerrado. A set of 4,422 candidate genes was found in roots and leaves. The number of down-regulated transcripts in roots was higher than the up-regulated transcripts, while the opposite occurred in leaves. The number of common transcripts between the two tissues was 1,249, while 2,124 were specific to roots and 1,049 specific to leaves. Quantitative RT-PCR analysis revealed a 0.78 correlation with the expression data. The candidate genes were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 454 sequencing; Candidate genes; RT-qPCR; Triticum aestivum; Water deficit.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400629
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Glutenin analysis for the safe screening of self-pollinated wheat seeds when developing doubled-haploid populations PAB
Torres,Gisele Abigail Montan; Scagliusi,Sandra Maria Mansur; Chaves,Márcia Soares; Consoli,Luciano.
Abstract: The objective of this work was to apply glutenin analysis, as a simple and reliable method, to identify self-pollinated wheat (Triticum aestivum) seeds in F1 crosses, when developing mapping populations using doubled-haploid (DH) technology. F1 seeds were subjected to glutenin analyses using gel electrophoresis, to verify and confirm their heterozygotic profiles. Glutenin analyses were carried out using one half of the endosperm of each of the 87 F1 seeds. The other half of the endosperm was used to develop the mapping population through DHs. Plants with band patterns different from the expected ones were discarded. Although the incidence of self-pollinated seeds was relatively low (only 8%), the screening of F1 heterozygotic profiles is...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Puccinia recondita f. sp. tritici; Triticum aestivum; Adult plant resistance; Leaf rust; Microspore culture; Toropi wheat cultivar.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000500646
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Impact of high-molecular-weight glutenin alleles on wheat technological quality PAB
Vancini,Camila; Torres,Gisele Abigail Montan; Miranda,Martha Zavariz de; Consoli,Luciano; Bonow,Sandro; Grando,Magali Ferrari.
Abstract: The objective of this work was to determine high-molecular-weight glutenin subunits (HMW-GS) and their relationship with technological quality parameters in a collection of wheat (Triticum aestivum) genotypes. Two hundred and seventy-two accessions were evaluated on SDS-Page, and molecular markers were used to identify the 7oe allele and 1BL.1RS translocation. For 219 accessions with a homogenous glutenin profile, 53 profiles and 21 alleles were identified. The most frequent combination was 2*/7+9/5+10 (11.9%). The mean value of genetic diversity for the three assessed Glu-1 loci was 0.67. Based on the HMW-GS profile and on the presence of the 1BL.1RS translocation, the Glu-1 score was calculated and its correlation with technological quality...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Triticum aestivum; Bread-making quality; Glu-1 score; Gluten strength; HMW-GS.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2019000102801
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Potato cultivar identification using molecular markers PAB
Rosa,Patricia Marchezi; Campos,Tatiana de; Sousa,Adna Cristina Barbosa de; Sforça,Danilo Augusto; Torres,Gisele Abigail Montan; Souza,Anete Pereira de.
The objective of this work was to evaluate a set of microsatellite markers for varietal identification and characterization of the most widespread potato cultivars in Brazil. The DNA from 14 potato cultivars was genotyped using microsatellite markers and the alleles were scored in silver-stained polyacrylamide gel. Twenty-four microsatellite markers were evaluated, and only one locus was monomorphic. Based on band patterns, a set of two microsatellites that were able to identify and differentiate all examined cultivars was obtained.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Solanum; AFLP; Genetic certification; Varietal protection; Variety identification; RAPD.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010000100015
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Sowing date reduces the incidence of wheat blast disease PAB
Coelho,Maurício Antônio de Oliveira; Torres,Gisele Abigail Montan; Cecon,Paulo Roberto; Santana,Flávio Martins.
Abstract: The objective of this work was to assess the effect of sowing date on the intensity of wheat blast disease, as well as the yield losses caused by this disease in different wheat (Triticum aestivum) genotypes. The experiments were conducted in 2013 at the Sertãozinho experimental station of Empresa de Pesquisa Agropecuária de Minas Gerais (Epamig), in the municipality of Patos de Minas, in the state of Minas Gerais, Brazil. Fourteen wheat genotypes and two sowing dates were evaluated. The experimental design was a randomized complete block with three replicates. The evaluated variables were: incidence, severity, thousand grain weight (TGW), grain yield, and yield losses. A disease index (DI) was calculated, based both on the incidence and the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Magnaporthe oryzae; Pyricularia oryzae; Triticum aestivum; Disease index; Severity; Tolerance..
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2016000500631
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